pathscan® intracellular signaling array kit Search Results


pathscan® intracellular signaling array kit (fluorescent readout) #7744  (EuroClone)
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EuroClone pathscan® intracellular signaling array kit (fluorescent readout) #7744
Phosphorylation of ERK1/2, SAPK/JUNK, and p38 in mesothelioma cells (MES). (A–C) MES were allowed to adhere to hyaluronic acid (HA) or HA-bound-C1q and the phosphorylation status of ERK1/2, SAPK/JUNK, and p38 was evaluated using total cell lysates with <t>PathScan</t> Antibody Array Kit (Cell Signaling), as described in the Section “ .” Data from at least three independent experiments are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 (Student’s t -test). (D) Three different populations of MES were labeled with the fluorescent dye FAST DiI, preincubated for 30 min at 37°C with P38, ERK, or JNK inhibitors and then allowed to adhere to microtiter wells precoated with HA-bound-C1q. The data are expressed as mean of three independent experiments done in triplicates ± SEM. *** p < 0.001 vs. resting.
Pathscan® Intracellular Signaling Array Kit (Fluorescent Readout) #7744, supplied by EuroClone, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pathscan® intracellular signaling array kit (fluorescent readout) #7744 - by Bioz Stars, 2026-03
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Phosphorylation of ERK1/2, SAPK/JUNK, and p38 in mesothelioma cells (MES). (A–C) MES were allowed to adhere to hyaluronic acid (HA) or HA-bound-C1q and the phosphorylation status of ERK1/2, SAPK/JUNK, and p38 was evaluated using total cell lysates with PathScan Antibody Array Kit (Cell Signaling), as described in the Section “ .” Data from at least three independent experiments are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 (Student’s t -test). (D) Three different populations of MES were labeled with the fluorescent dye FAST DiI, preincubated for 30 min at 37°C with P38, ERK, or JNK inhibitors and then allowed to adhere to microtiter wells precoated with HA-bound-C1q. The data are expressed as mean of three independent experiments done in triplicates ± SEM. *** p < 0.001 vs. resting.

Journal: Frontiers in Immunology

Article Title: Complement Protein C1q Binds to Hyaluronic Acid in the Malignant Pleural Mesothelioma Microenvironment and Promotes Tumor Growth

doi: 10.3389/fimmu.2017.01559

Figure Lengend Snippet: Phosphorylation of ERK1/2, SAPK/JUNK, and p38 in mesothelioma cells (MES). (A–C) MES were allowed to adhere to hyaluronic acid (HA) or HA-bound-C1q and the phosphorylation status of ERK1/2, SAPK/JUNK, and p38 was evaluated using total cell lysates with PathScan Antibody Array Kit (Cell Signaling), as described in the Section “ .” Data from at least three independent experiments are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 (Student’s t -test). (D) Three different populations of MES were labeled with the fluorescent dye FAST DiI, preincubated for 30 min at 37°C with P38, ERK, or JNK inhibitors and then allowed to adhere to microtiter wells precoated with HA-bound-C1q. The data are expressed as mean of three independent experiments done in triplicates ± SEM. *** p < 0.001 vs. resting.

Article Snippet: Pathway analysis was performed as per the manufacturer’s instructions of the PathScan ® Intracellular Signaling Array Kit (Fluorescent Readout) (#7744; Cell Signaling Technology, EuroClone, Milan, Italy).

Techniques: Phospho-proteomics, Ab Array, Labeling